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Part:BBa_K1878005:Experience

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


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UNIQf532e081a76e28b4-partinfo-00000000-QINU UNIQf532e081a76e28b4-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.

Fig 1. Results of part assay in aerobic, and anaerobic, conditions.